Fig. 3
Effects of avocado peel and seed extracts on MMP-1 and type I collagen levels in UVB-irradiated BJ cells. The cells were pre-treated with 12.5–50 µg/ml of the extracts, irradiated with 40 mJ/cm2 UVB, maintained in the culture medium for 24 h, and determined the levels of MMP-1 proteins by Western blot analysis in (A) avocado peel extract and (B) avocado seed extract, and (C) determined the collagen content in avocado peel and seed extracts-treated cells in the supernatants by using ELISA after UVB irradiation 24 h. The results are presented as the mean ± SEM of three independent experiments. The statistical significance of differences was evaluated by one-way ANOVA followed by Tukey’s test. **p < 0.01 compared to the non-irradiated control; #p < 0.05, ##p < 0.01 compared to the UVB-irradiated control.
