Fig. 2

Representative two-photon images of esophageal cancer tissues from patients diagnosed with SCC at the first diagnosis. (a) H&E-stained histological image showing the layered structures of esophageal tissue. (b)-(f) show five image sets. Merge indicates the overlaying of SHG and TPF images. Dashed lines represent layer boundaries. Image size: 1.6 × 1.6 mm2.