Fig. 5

Representative two-photon images of esophageal cancer tissues from patients diagnosed with HGD for the second diagnosis (as the follow-up 6 months after the first diagnosis shown in Fig. 4). (a)-(e) show five image sets. Merge indicates the overlaying of SHG and TPF images. Dashed lines represent layer boundaries. Image size: 1.6 × 1.6 mm2.