Fig. 2
Transcriptome analysis of eight sex- and age-balanced BM-MSC preparations by RNA-seq. (A) Volcano plot emphasizing differentially expressed genes with DESeq2-based padj-value cut-off 0.05. These genes were included in the gene set enrichment analysis in C, D. Arrows highlight GPNMB and SDC4. (B) Heatmap showing differentially expressed genes with DESeq2-based padj-value cut-off 0.05. Qlucore Omics Explorer was used for the heatmap visualisation and normalization of the data (each column has mean of 0 and variance of 1) of four primary hip surgeries vs. four revisions (2x hip, 2x knee). Arrows highlight GPNMB and SDC4. (C) KEGG analyses (KEGG: https://www.kegg.jp/;14 of downregulated genes (upper panel) as identified using DESeq2. Gene ontologies for Biological Processes (middle panel) and Molecular Functions (lower panel) are included. (D) KEGG analyses of upregulated genes (upper panel) as identified using DESeq2. Gene ontologies for Biological Processes (middle panel) and Molecular Functions (lower panel) are included.
