Fig. 7
Transcriptome analysis of all primary vs. all revision arthroplasties at both implantation sites (28 samples). (A) KEGG analyses (KEGG: https://www.kegg.jp/;14 of downregulated genes (upper panel). Gene ontologies for Biological Processes (middle panel) and Molecular Functions (lower panel) are included. (B) KEGG analyses of upregulated genes (upper panel). Gene ontologies for Biological Processes (middle panel) and Molecular Functions (lower panel) are included. (C) RNA-seq data for GPNMB in BM-MSCs (normalised counts). For the statistical comparisons between primary and revision the parametric unpaired Welch’s t-test was used. Experimental data are shown as individual values. (D, E) GPNMB relative expression levels in BM-MSCs based on RPS29 as housekeeping gene. E indicates the levels for individual samples. (F) GPNMB relative expression levels in MNCs from blood (PB) and synovial fluid (SF). (G) RNA-seq data for SDC4 in BM-MSCs (normalised counts). For the statistical comparisons between primary and revision the parametric unpaired Welch’s t-test was used. Experimental data are shown as individual values. (H, I) SDC4 relative expression levels in BM-MSCs based on RPS29 as housekeeping gene. I indicates the levels for individual samples. (J) SDC4 relative expression levels in MNCs from blood (PB) and synovial fluid (SF). The black bar in C, D, G, H represents the median value. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
