Fig. 3
From: Harnessing prebiotic formamide chemistry: a novel platform for antiviral exploration
Overlaid chromatograms (254 nm) from PAMPA assay showing the composition of donator compartment (violet), acceptor compartment (blue) and equilibrium (green) over time. The analysis was performed by LC-UV/MS on a Zorbax Eclipse XDB-C18 column (250 × 4.6 mm, 5 μm) using a binary gradient of H₂O+ (0.1% formic acid) and ACN/MeOH (1:1, v/v) at a flow rate of 0.6 mL/min; structures of the main components obtained by partial deconvolution are shown; Donor Compartment represents the remaining compound in the donor side at the end of the experiment; Acceptor Compartment represents compounds that permeated through the artificial membrane into the acceptor side; Equilibrium represents the equilibrium concentration where donor and acceptor compartments would reach equal concentrations. Insert table on the left shows Papp values and membrane retention expressed as percentage of compound unable to reach the acceptor compartment for peaks A-E.
