Fig. 6 | Scientific Reports

Fig. 6

From: Glucose stimulated CEST MRI pHe mapping for improved differentiation of tumors with altered hypoxia inducible factor 1alpha expression

Fig. 6

(a) Western blot displaying LDHA protein expression in 231-WT, 231-HIF-1α- shRNA and 231-VEGF tumors. GAPDH was used as a loading control. (b) Quantification of LDHA expression in tumor lysates. Bar graph showing relative LDHA protein expression levels in tumors derived from 231-WT, 231-HIF-1α-shRNA, and 231-VEGF cell lines. Protein expression was quantified from Western blot band intensities and normalized to loading control. (Values represent Mean ± S.E.M., *P < 0.05 and **P < 0.01, 231-WT: n = 9; 231-HIF-1α-shRNA: n = 7; 231-VEGF: n = 4). Merged bright-field and chemiluminescence images of LDHA and the corresponding GAPDH immunoblots are presented in Fig. S3 of Supplementary Information.

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