Fig. 5

Comparison of key parameters between age groups. Boxplots showing the distribution of (a) number of fiber clusters, (b) fiber diameter, (c) normalized skin firmness, and (d) maximum cluster size for the Younger/Middle-Aged (38-49 years; n=4) and Older (58-78 years; n=5) cohorts. The boxes represent the interquartile range (IQR), the horizontal line is the median, and the whiskers extend to 1.5 times the IQR. The results show a significant increase in fragmentation (a) and decrease in fiber diameter (b) in the older cohort, with corresponding trends of declining skin firmness (c) and network integrity (d).