Fig. 3
Effects of CS on Erk1/2 and phospho-Erk1/2 in the brain of rats with fluorosis. (A) Immunohistochemistry analysis was utilized to assess the expression levels of phospho-Erk1/2 protein. The representative images labeled as a1-c1, a2-c2, and a3-c3 correspond to the parietal cortex, the CA2 region, and the CA3 region, respectively. Bar = 50 μm. (B) Mean optical density values of phospho-Erk1/2 in the parietal cortex, the CA2 region and the CA3 regions. (C) Representative images of Western blot for Erk1/2 and phospho-Erk1/2 in brain. Quantitative analyses of Erk1/2 (D), phospho-Erk1/2 (E) protein and the ratio of phosphorylated Erk1/2 to total Erk1/2 expression levels (F) normalized to the internal control α-Tublin. The samples derive from the same experiment and that blots were processed in parallel. Original blots are presented in Supplementary Fig. 2. Data are shown as the mean ± SEM; n = 6 for each group. *P < 0.05 with the control group. #P < 0.05 with the Fluorie group. nsP > 0.05 .
