Fig. 4
Effects of CS on MMP-2 and MMP-9 in the brain of rats with fluorosis. The expression levels of MMP-2 protein (A) and MMP-9 protein (C) was detected by immunohistochemistry analysis. The representative samples labeled as a1-c1, a2-c2 and a3-c3 correspond to the parietal cortex, the CA2 region and the CA3 region, respectively. Bar = 50 μm. Mean optical density values of MMP-2 (B) and MMP-9 (D). Representative images of Western blot for MMP-2 protein and MMP-9 protein in brain (E). Quantitative analyses of MMP-2 (F) and MMP-9 (G) protein expression levels normalized to the internal control α-Tublin. The samples derive from the same experiment and that blots were processed in parallel. Original blots are presented in Supplementary Fig. 3. Data are shown as the mean ± SEM; n = 6 for each group. * P < 0.05 with the control group. #P < 0.05 with the Fluorie group.
