Fig. 5
The Erk1/2/MMP-2/MMP-9 signaling pathway inhibition reversed synaptic changes induced by fluoride in SH-SY5Y cells. (A) CCK-8 assay was detected to cell viability of SH-SY5Y cells treated with the different concentrations of PD98059 (0, 5, 10, 20, 30 µmol/L) for 48 h. (B) Quantification of PD98059-mediated suppression of Erk1/2 gene expression by RT-qPCR. (C) The cell viability of SH-SY5Y cells among three groups rats after PD98059 (10 µmol/L) treatment for 48 h. (D) The immunofluorescence staining and (E) quantification analysis of SYN protein in SH-SY5Y cells. (F) The protein levels of MMP-2, MMP-9 and SYN were examined by Western blot in SH-SY5Y cells. (G) Quantitative analyses of MMP-2, MMP-9 and SYN protein expression levels normalized to the internal control α-Tublin. (H) RT-qPCR was used to measure the expression levels MMP-2, MMP-9 and SYN gene in SH-SY5Y cells. The samples derive from the same experiment and that blots were processed in parallel. Original blots are presented in Supplementary Fig. 4. Data are shown as the mean ± SEM; n = 3 for each group. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 with the control group. #P < 0.05 and ##P < 0.01 with the Fluorie group.
