Fig. 6
CS suppressed synaptic damage induced by fluoride in SH-SY5Y cells. (A) Cell viability of SH-SY5Y cells treated with the CS of different concentrations (0.2, 0.4, 0.6, 0.8 mg/L) for 48 h were detected by CCK-8 assay. (B) CCK-8 was used to examine the cell viability of SH-SY5Y cells after CS (0.4 mg/ml) treatment for 48 h. (C) The Western blot and (D) quantification analysis of Erk1/2, phospho-Erk1/2, MMP-2, MMP-9 and SYN in SH-SY5Y cells. (E) The ratio of phosphorylated Erk1/2 to total Erk1/2 expression levels. (F) RT-qPCR analysis of Erk1/2, MMP-2, MMP-9 and SYN gene in SH-SY5Y cells. (G) The immunofluorescence staining and (H) quantification analysis of SYN in SH-SY5Y cells. (I) Ultrastructural observation of the SH-SY5Y cells. Red arrows: chromatin condensation and margination. Red box: integrity of the nuclear membrane disappeared. Yellow box: The endocytosis vesicles. Bar = 10 μm. The samples derive from the same experiment and that blots were processed in parallel. Original blots are presented in Supplementary Fig. 5. Data are shown as the mean ± SEM; n = 3 for each group. * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001. nsP > 0.05.
