Fig. 1
From: Effect of ultrasound-microbubble exposure on acute myeloid leukemia cancer cell proteome
Schematic diagram of the experimental design and analysis. Flow cytometry (left branch) was used to measure the uptake of FITC and PI at US (950 kPa) and USMB (0-950 kPa). Following USMB with FITC, the sample was incubated (37 °C) for 30 min. PI was added prior to analysis of cells with flow cytometry. Cells prepared and treated simultaneously and split for MS and WB analysis (center and right branch). MS and WB cells went through their respective digestions and preparation for analysis. TMT labelling was done for analysis through MS. WB lysates were prepared for immunoblotting.
