Fig. 6

(A) Radar graph of GO terms generated by running DAVID Bioinformatics with DEP₇₈ and statistically identified proteins, separately. Statistically identified proteins correspond to Detected Proteins which were discovered to be statistically significant compared to the untreated control. Values on the chart correspond to the percentage of proteins from a particular DAVID analysis, which are mapped to a particular GO term. GO terms were included if they had a p-value of ≤ 0.05 and ≥ 5% of proteins from their respective lists attributed to them. (B) Radar graph of GO terms generated by running DAVID Bioinformatics with DEPs for each USMB condition separately (DEPs listed in Table S1). Values on the chart correspond to the percentage of proteins from a particular DAVID analysis, which are mapped to a particular GO term. GO terms were selected if they had a p-value of ≤ 0.05 and ≥ 5% of proteins from their respective lists attributed to them. C) A heatmap with the combined results of DAVID Bioinformatics, run with DEPs for each USMB condition. DEPs of interest (Table 1) from the USMB_450kPa,6 h (DEP14), USMB_750kPa,6 h and USMB_750,24 h conditions were entered into DAVID Bioinformatics separately; a list of GO terms was generated for each group of DEPs. GO results were counted as “hits” if they had a p-value of ≤ 0.05 and if the number of proteins in each list attributed to a particular GO term was ≥ 5%. Results for each condition were plotted on one heatmap; overlapping hits are denoted by unique colours. (AJ – Adherens Junction; APM – Apical Plasma Membrane; BM – Blood Microparticle; ER – Endoplasmic Reticulum; ES – Extracellular Space; FA – Focal Adhesion; GA – Golgi Apparatus; IMBO – Intracellular Membrane-Bounded Organelle; PRC – Perinuclear Region of Cytoplasm; PM – Plasma Membrane; RNAP2TRC – RNA Polymerase II Transcription Regulator Complex).