Fig. 2
Compound modulation shifts a SNCA-4x towards a control morphological profile. (A) Overview about screening library composition and neuron treatment scheme. (B) Schematic overview of image data generation. Morphological features were calculated from imaging data to measure compound-induced morphological profile changes from a SNCA-4x towards a SNCA-corr-like profile. (C) Schematic of morphological profile data processing workflow. (D) Overview of morphological feature types used for hit selection after removal of redundant features using a Pearson correlation threshold of 0.95. (E) Morphological profile Mahalanobis distances of compound treated mDA neurons relative to SNCA-4x and SNCA-corr morphological profiles. The black dashed line indicates the selection window based on 4σ from the SNCA-4x median. Each datapoint represents mean data from one well and for compounds the mean value of all four replicates is shown. (F) Compounds inducing morphological profiles with the 10 shortest mean Mahalanobis distances to the median SNCA-corr morphological profile. (G) Selected hits clustered by the Cosine similarity of their respective morphological profiles. Compounds are color coded by attributed biological activity. (H) Tanimoto similarities of top 10 selected compounds.
