Fig. 2 | Scientific Reports

Fig. 2

From: Extremely low-frequency electromagnetic fields facilitate proliferation and functional differentiation in spinal neural stem cells

Fig. 2

ELF-EMFs enhance proliferation and self-renewal of spinal cord-derived NSCs. A Representative confocal images showing the proliferation of the spinal cord-derived NSCs stained with Ki67 (green) after exposure to ELF-EMFs at different intensities. Scale bar, 100 μm. Insets display magnified view of the boxed region. B Quantification of Ki67+ cell proportion (n = 4). Values are normalized to the 0 mT control group. C, D Representative images showing self-renewal through primary and secondary neurosphere formation assays after exposure to ELF-EMFs at different intensities. Scale bar, 100 μm. E, F Quantification of the primary (n = 4) and secondary (n = 4) neurosphere formation rates. Values are normalized to the 0 mT control group. G Quantification of Sox2 mRNA levels in spinal cord-derived NSCs after exposure to ELF-EMFs at different intensities (n = 5). Values are normalized to the 0 mT control group.

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