Table 2 Primers used in qRT-PCR analyses.
Gene | Forward primer | Reverse primer |
|---|---|---|
Sox2 | 5’-AACCGATGCACCGCTACGA-3’ | 5’-TGCTGCGAGTAGGACATGCTG-3’ |
NeuN | 5’-CCACGCTTCTCCTTGTCTCG-3’ | 5’-CCTTGAAGCCAGCATTGAGC-3’ |
NeuroD1 | 5’-ACAACAGGAAGTGGAAACATGACC-3’ | 5’-CACTCATCTGTCCAGCTTGGG-3’ |
NeuroD2 | 5’-CCAAGCCAGTGTCTCTTC-3’ | 5’-CTTCCTCCTCCTCCTCTTC-3’ |
NeuroG1 | 5’-CCAGCGACACTGAGTCCTG-3’ | 5’-CGGGCCATAGGTGAAGTCTT-3’ |
NeuroG2 | 5’-GTCATCCTCCAACTCCACGTC-3’ | 5’-AGGCGCATAACGATGCTTCTC-3’ |
Cav1.2 | 5’- CGCAGCGTAAGGATGAGTGA-3’ | 5’- ACCTAGAGAGGCAGAGCGAA-3’ |
Cav1.3 | 5’- GCTCCCACCTCAACAAATGC-3’ | 5’- TGCTTGCAGGAGTAATGCCC-3’ |
Cav3.1 | 5’- GCTCTACTTCATCTCCTTCC-3’ | 5’- GCTTCTCCTTACTCCTTCTC-3’ |
Cav3.2 | 5’- GCTCTTCATCTTCATCTTCAG-3’ | 5’- AACCACATTCCAGTCTTCC-3’ |
Cav3.3 | 5’- GCACTACAACCAGCCTAC-3’ | 5’- GACAGACAGCAGCACAAT-3’ |
GFAP | 5’-AGCCAAGCAGCCCACCAAAC-3’ | 5’-TCTATACGCAGCCAGGTTGTTCTC-3’ |
GAPDH | 5’-ATACGGCTACAGCAACAGGG-3’ | 5’-GCCTCTCTTGCTCAGTGTCC-3’ |
