Table 1 VEGF concentrations obtained for the positive and negative controls for the 11 series performed to validate the VEGF quantification method in patient supernatants using the ELLA system and its VEGF cartridge, four series to assess the linearity parameter and six for the precision. *Indicates that concentration is out of range.

From: Validation of a potency assay for CD34 + cell based therapy

 

Series

Positive control high

Positive control low

Negative control

Range 1108–2274

pg/mL

Range 24.4–42.0

pg/mL

< LLOQ

Linearity Data

Day 1

Serie 1a

1482

31

1*

Serie 1b

1630

32

1*

Serie 1c

1491

30

0*

Day 2

Serie 2

1495

29

1*

Serie 3

1557

31

1*

Precision Data

Day 1

Serie 1

1559

31

0*

Serie 4

1572

32

0*

Day 2

Serie 2

1270

28

1*

Serie 5

1330

27

2*

Day 3

Serie 3

1602

33

2*

Serie 6

1941

35

2*

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