Table 6 Summary of analysis of variance and variance components using adjusted sum of squared. ANOVA (general linear model) was performed by minitab including “cartridge batch” and “operators” as factors and the associated interaction. *Value is negative and is estimated by zero. These results have been normalized to the number of CD34 + cells obtained after expansion and can be found in supplemental table 2.
From: Validation of a potency assay for CD34 + cell based therapy
Analysis of Variance | Patient 045 | Patient 052 | Patient 056 | Patient 065 | ||||
|---|---|---|---|---|---|---|---|---|
Source | DF | P-Value | DF | P-Value | DF | P-Value | DF | P-Value |
Cartridge Batch | 2 | 0.03 | 2 | 0.04 | 2 | 0.01 | 2 | 0.08 |
Operator | 1 | 0.84 | 1 | 0.28 | 1 | 0.10 | 1 | 0.84 |
Cartridge Batch*Op | 2 | 0.13 | 2 | 0.03 | 2 | 0.22 | 2 | 0.00 |
Error | 12 | 11 | 12 | 12 | ||||
Total | 17 | 16 | 17 | 17 | ||||
Variance Components, using Adjusted SS | Patient 045 | Patient 052 | Patient 056 | Patient 065 | ||||
Source | Variance | % of Total | Variance | % of Total | Variance | % of Total | Variance | % of Total |
Cartridge Batch | 97199.6 | 90% | 757.4 | 88% | 14510.2 | 89% | 31480.9 | 83% |
Operator | -1813.91* | 0% | 22.7 | 3% | 985.9 | 6% | -1779.11* | 0% |
Cartridge Batch*Op | 3395.9 | 3% | 47.2 | 5% | 167.2 | 1% | 5220.2 | 14% |
Error | 7091.0 | 7% | 33.7 | 4% | 707.3 | 4% | 1226.5 | 3% |
Total | 107687.0 | 861.0 | 16370.6 | 37927.6 | ||||
