Fig. 4
From: Morphology of the glymphatic and meningeal lymphatic structures of the bottlenose dolphin
Light and confocal micrographs representing a transverse section of the bottlenose dolphin (Tursiops truncatus) superior sagittal sinus displaying the histomorphology of the parasagittal structures stained with Hematoxylin and Eosin and confirmed using immunofluorescent markers PROX-1 and VEGFR3. Scale bars represent 1Ā mm (A) and 50Ā Ī¼m (B ā H). (A) Composite photomicrograph of histological section demonstrating the structural arrangement of the superior sagittal sinus (SSS) in transverse section (JAX 002). Periosteal (PDL) and meningeal (MDL) dural layers, falx cerebri (FC), subarachnoid space (SAS), and cerebral hemispheres (CH) shown. Boxes correspond to the sites of (B) and (C). (B) Photomicrograph of the simple endothelial layer that lines the dorsal and ventral aspects of the SSS lumen. (C) Photomicrograph of blood vessels (BV) (arrows), in this case dorsal cerebral veins, that extend from the brain into the parasagittal dura and drain directly into SSS. Dorsal cerebral veins have a smooth muscle layer that merges with the lateral wall of SSS, which differs from the simple endothelium shown in (B). Meningeal lymphatic vessels (largest labeled MLV, others marked by arrowheads) are also present within the dura mater. Box corresponds to site of (D)-(H). (D ā H) Representative confocal images (individual and merged) demonstrating and differentiating the presence and localization of blood and lymphatic vessels using immunofluorescent markers for vascular endothelial growth factor receptor 3 (VEGFR3) (green), and Prospero homeobox protein 1 (PROX-1) (magenta), with DAPI (white) counter labeling for nuclei. Note that red blood cells are auto fluorescent. Individually labeled confocal images are shown to illustrate VEGFR3 and PROX-1 localization, which can be obscured in the merged image. Arrows represent dorsal cerebral veins and arrowheads represent MLVs.
