Fig. 7
From: Morphology of the glymphatic and meningeal lymphatic structures of the bottlenose dolphin
Photomicrographs of histological sections of brain parenchyma of the bottlenose dolphin (Tursiops truncatus) stained with Hematoxylin and Eosin and immunofluorescent markers for displaying the morphology of perivascular spaces and the presence of aquaporin-4 water channels lining, penetrating blood vessels. Scale bars represent 100Ā Ī¼m. (A) KLC 360; (B) TFK 020; (C) TFK 013; (D-E) KLC 360 (F) TFK 020 (G) KLC 360 (H) TFK 020 (I) TFK 013 (J) KLC 360 (K) TFK 020 (L) KLC 360. (A) Composite light micrograph demonstrating the perivascular space (PVS) (arrows) surrounding blood vessels penetrating the brain parenchyma. Larger pial blood vessels adjacent to the brain are labeled BV. (B ā C) Individual light micrographs demonstrating the presence and annular morphology of PVS (arrows) within the cerebrum of two individual dolphins. (D) PVS (arrows) surrounding blood vessels penetrating the gray matter and (E) white matter of the cerebellum. (F ā L) photomicrographs demonstrating immunoreactivity of the astrocyte marker glial fibrillary acidic protein (GFAP, magenta) and astroglial water channel aquaporin-4 (AQP4, green) are shown surrounding large lectin (red) positive vessels in representative standard full focus z-stack micrographs of dolphin brain tissue. (F, G) Representative images of PVSs (arrows) surrounding penetrating vessels at the brain surface are shown. (H ā L) Widespread astrocytic AQP4 expression is observed throughout the brain with enrichment at astrocyte end-feet surrounding blood vessels and associated PVSs (arrows).
