Fig. 2
Interpretation of the cryo-EM density map for Pd-CI-cNDs. a The 3.1 Å-resolution cryo-EM density map of Pd-CI in cNDs coloured by subunit and presented within the Gaussian-smoothed large phospholipid cND bilayer (purple). b Comparison of the Pd-CI-cND map and the Pd-CI-2N2-ND map from Ivanov et al.19 used to determine the high-resolution structure of Pd-CI. The membrane-embedded portion of Pd-CI is coloured red and the hydrophilic domain green. c Comparison of Pd-CI in the cND with the hybrid DDM:2N2-ND bicelle observed in the 2N2-ND preparation. d Footprint comparison of Pd-CI to 2N2 and cND, with approximate maximum ‘diameters’ of length and width based on the map densities. Cryo-EM density maps were visualized using UCSF ChimeraX57 v1.6 [https://www.rbvi.ucsf.edu/chimerax/].
