Fig. 4
From: DNA aptamer AptERA 2 targets ERA from Staphylococcus aureus and limits GTP hydrolysis
AptERA 2 binding analysis under ex vivo conditions. (a) Structural dependence for AptERA 2 binding to S. aureus ERA in E. coli lysates expressing the protein. Native and 2% SDS-treated lysates were compared. Mean ± s.d. from triplicate experiments are shown. Statistical analysis was performed using T-test (**P = 0,017). (b) Binding specificity of AptERA 2 to ERA, RbgA, and 1F8 using total lysates of overexpressing E. coli BL21 strains. Mean ± s.d. from triplicate experiments are shown. Statistical analysis was performed using one-way ANOVA (****P ≤ 0.0001). (c) Binding signal dependence on AptERA 2 concentration in E. coli lysates. The absorbance signal (450 nm) in the Y axis was corrected by subtracting a non-aptamer blank for all measurements. The continuous line shows the fitting with hyperbolic function for binding (Kd = 884 ± 58 nM).
