Fig. 9

Effect of pre-injection with Yps proteins on the survival of G. mellonella larvae infected with C. glabrata. Larvae were injected with 1 µg of Yps3 or Yps9 in 10 µl, and 48 h later were challenged with two different doses of the pathogen ((A) 1.25 × 10⁶ or (B) 2.5 × 10⁶ CFU per larva). A dashed vertical line on the graph indicates the time point of pathogen injection (48 h post Yps-administration). Representative data are shown in the graph, with the experiment performed in three independent biological replicates, each group containing ten larvae. Survival rates were compared with the control group that received the pathogen without prior exposure to Yps. Larvae injected with DPBS served as an additional control.