Table 1 Primer sequences were utilized for gene expression analysis using quantitative PCR.
Gene name | Symbol | Left primer | Right primer |
|---|---|---|---|
Citrate synthase | CS | AGAAACTGCTACCCAAGGCT | GGGGTGTAGATTGGTGGGAA |
Aconitase 2 | ACO2 | ATGAAGATATGGGGCGCTCA | ATGTCCTTCCTGTCCCACTG |
Isocitrate dehydrogenase 2 | IDH2 | GGCTCAGGTCCTCAAGTCTT | AGCCTCAATCGTCTTCCCAT |
Oxoglutarate (α-ketoglutarate) dehydrogenase | OGDH | TTTGGTCTAGAAGGCTGCGA | TCTGTTCCAGCTCCTTCCTG |
Dihydrolipoamide S-succinyltransferase | DLST | GCAAATGGCGTGATTGAAGC | ATGCTCAGTCCCAATCCCAA |
Succinate-CoA ligase GDP-forming subunit β | SUCLG2 | CCTGCTTTGTGAATGGTGCT | GCACAGTTGACGATACCACC |
Succinate dehydrogenase complex subunit C | SDHC | CCTACTCTCGGCCTAGAAGC | GTCCTTTAAGCCAGCAACCC |
Fumarate hydratase | FH | CCACTTACTCTTGGGCAGGA | GGAGCAGTGACAAAAGGCAA |
Malate dehydrogenase 2 | MDH2 | CAACGTCCCTGTCATTGGTG | CAAGGGAGAAGACAAAGCGG |
Pyruvate dehydrogenase α1 | PDHA1 | TGTGTGATGGTCAGGAAGCT | ACATGTGCATCGATCCTCCT |
Glutamic-oxaloacetic transaminase 2 | GOT2 | AAGTAATGTCCCTGGTGCGA | CTCTGCCCTGTGTCACTACA |
Glutaminase | GLS | GATTGCGAACGTCTGATCCC | TGCAACCTTTCCTCCAGACT |
Glutamic-pyruvic transaminase 2 | GPT2 | TGCTGTGCTGTTTCCATCTC | TTTGGCAGGAATGAAGATCC |
Glutamic-pyruvic transaminase | GPT | AAGGGCTGAGGGGTTAGGTA | AAGACCTGGGAAGACGGAGT |
β-actin | ACT-B | ATAGCACAGCCTGGATAGCAA | AGGATTCCTATGTGGGCGAC |
