Fig. 6
From: Uncovering novel functions of NUF2 in glioblastoma and MRI-based expression prediction
NUF2 regulates GBM cell proliferation and migration through β-catenin and MMP9. (A) Scratch assay showing the migration results of shNC-U251 and shNUF2-U251 cells treated with Licl (0 mM, 10 mM) at 0 h and 24 h, and quantification of the wound healing ratio. (B) After treatment with 10 mM LiCl, the number of U87 and U251 cells transfected with shNC or shNUF2 decreased, but the vitality inhibition caused by NUF2 knockdown was reversed. (C) Cell viability assay of U87 and U251 cells transfected with shNC or shNUF2 and treated with DMSO or 250 µM TMZ showed that NUF2 knockdown increased sensitivity to TMZ treatment. (D) Western blot analysis of NUF2, β-catenin, and MMP9 protein levels in U87 and U251 cells transfected with shNC or shNUF2, along with quantification of the relative protein levels of NUF2, β-catenin, and MMP9, indicated that NUF2 expression was positively correlated with β-catenin and MMP9. *p < 0.05, **p < 0.01, and ***p < 0.001.
