Fig. 2
Muscone alleviates the cell apoptosis and impaired cell cycle of NP cells induced by IL-1β. (A) Annexin V-FITC/PI Double-staining apoptosis assay showing the percentage of apoptotic percentage of NP cell under IL-1β and IL-1β + muscone treatment. (B) The mean percentage of early apoptotic cells and late apoptotic cells in each group was shown in the right panel. (C) The cell apoptosis of NP cells under TNF-α and TNF-α + muscone treatment was measured by flow cytom-etry. (D) The mean percentage of early apoptotic cells and late apoptotic cells in each group was shown in the right panel. TUNEL assay was used to measure cell apoptosis of NP cells under IL-1β (E) and TNF-α (F) treatment, bar represents 100 μm. The flow cytometry showing the cell cycle of NP cells under IL-1β (G) and TNF-α (H) treatment. The number of cells in different cell cycle under IL-1β (I) and TNF-α (J) treatment were shown in the panel. All experiments were repeated 3 times. Data are shown as mean ± SD. *, P < 0.05; **, P < 0.01.
