Fig. 6
Muscone alleviates cell senescence and SASP phenotype in NP cells via p53 signalling. (A) qPCR analysis showing the relative mRNA expression level of SASP factors, such as MMP13, MMP3, IL-6 and IL-8, in the NP cells, treat with or without SCH529074. (B,C) Western Blot analysis showing the protein levels of SASP factors in each group. (D) The relative mRNA level of p16INK4A and p21CIP expression was detected by qPCR in the NP cells. (E,F) Western Blot analysis showing the protein levels of p16INK4A and p21CIP in each group. (G) SA-b-gal staining was detected, and positive cells were quantified in (H), bar represents 200 μm. All experiments were repeated at least 3 times, and GAPDH is used as an internal control. Data are shown as mean ± SD. #, P < 0.05; ##, P < 0.01.*, P < 0.05; **, P < 0.01. ##represented vs. NC group, **represented vs. IL group.
