Fig. 3

Alterations in lung-resident immunity upon ovariectomy. (A) Schematic view of the experimental setup (created with Biorender). (B) Number of resident CD45⁺CD3^- cells in the lungs of ovariectomised (ox) and sham operated female mice. (C) Heatmap depicting the frequency of innate immune cells residing in the lungs of female ovariectomised (ox) mice normalised to the frequency of innate immune cells found in the lungs of female sham operated (sham) mice. (D–G) Frequency in resident CD45⁺CD3⁻ cells and number of resident (D) alveolar macrophages, (E) monocytes, (F) CD103⁺ DCs, and (G) neutrophils. (H) Heatmap depicting the frequency of adaptive immune cells residing in the lungs of ovariectomized mice normalised to the frequency of adaptive immune cells residing in the lungs of sham operated female mice. (I) Frequency in resident CD45⁺MHCII⁻ cells and number of resident CD4⁺ T cells. (J) Frequency in resident CD45⁺CD3⁻ cells and number of resident B cells. n = 5 mice per group. Each experiment was repeated twice. Data are shown as mean ± SEM. *: p ≤ 0.05, as assessed by Mann-Whitney test. Non-significant differences (p > 0.05) are stated as ns. res: resident; AM: alveolar macrophages; IM: interstitial macrophages; Mo: monocytes; DCs: dendritic cells; pDCs: plasmacytoid DCs; NKs: natural killer cells; TRMs: tissue-resident memory T cells.