Fig. 4
DHA ameliorates the locomotory defects in the l(2)gl/l(2)gl mutant at 250 µM. (A) Locomotion assay of third-instar larvae (wild-type, drug-treated, and untreated l(2)gl/l(2)gl mutants) showed rescue in the sluggish crawling movement (N = 20). (B) Age-related behavior assay (negative geotaxis) verified the rescue effect of DHA-treated newly eclosed adult flies with the mutation. Ten trials were conducted for this assay, with each trial noting the number of flies out of twenty flies that covered the required distance of 7.0 cm in 30 s. (C) Single fly behavior analysis done with age and sex-matched young adult flies (N = 10) with time as a parameter showed the almost similar time taken by the treated rescued flies and the wild-type flies to cross the predetermined 7.0 cm mark in the climbing apparatus (*p < 0.05, **p < 0.01, and ***p < 0.001).
