Fig. 5
Continuous and early DHA administration maintains the non-tumorigenic phenotype in l(2)gl/l(2)gl mutant strain. (A) Plot showing the effect of differential time-based drug exposure in Drosophila third-instar larvae on days 8, 9, and 10 post-egg laying, demonstrating early exposure (day 8) is required to elicit a significant anti-tumorigenic effect. (B) Schematic representation of the drug withdrawal-based generation experiment till the fourth generation. (C) Plot represents drug withdrawal’s effect on the brain area in the rescued l(2)gl/l(2)gl mutants (WD: Withdrawal of DHA) (N = 20). (D) Plot represents the effect of drug withdrawal on the wing pouch area in the imaginal discs in the rescued l(2)gl/l(2)gl mutants (N = 20) (*p < 0.05, **p < 0.01, and ***p < 0.001).
