Fig. 2
Macroscopic dissemination of tumor cells over time and blood 5-HT elevation in RV following intrahepatic B16F0-Tph1 implantation. (a) Representative images of mice injected with 1000 B16F0-Tph1 cells in the liver, showing progression of tumor spread at 5, 6 and 7 weeks post-injection. White arrows indicate extrahepatic dissemination (peritoneal carcinomatosis at 6 weeks and mediastinal lymph node metastases at 7 weeks). No extrahepatic dissemination was detected at 5 weeks, establishing this timepoint as optimal for the main study. (b) Blood 5-HT concentrations measured in the right ventricle (RV) and systemic circulation 5 weeks after intrahepatic implantation in B16F0-Tph1 and B16F0 mice. Data are presented as individual points with median ± IQR; n = 3 per group. Statistical significance was determined using Kolmogorov–Smirnov test (*p < 0.05 and **p < 0.01).
