Table 1 Primer system for the detection of MC4R gene SNP 732 locus.
From: Rapid SNP genotyping detection method based on PCR-lateral flow dipstick detection technique
Primers | Primer sequence (5ʹ → 3ʹ) | Modification method | Length |
|---|---|---|---|
MC4R-732-F-C | CGATCACCATCAGTGCCATCT | 5ʹ BIOTIN, LNA | 254 bp |
MC4R-732-F-G | CGATCACCATCAGTGCCATGT | 5ʹ BIOTIN, LNA | |
MC4R-R | GATCAGGATGGTCAGCGTGATC | 5ʹ FITC |
