Table 2 PCR amplification primer system.
From: Rapid SNP genotyping detection method based on PCR-lateral flow dipstick detection technique
Components | Volume (µL) |
|---|---|
Sheep whole blood | 2.00 |
2× Marathon buffer | 25.00 |
dNTP mixture (2.5 µM) | 10.00 |
PCR forward primer (10 µM) | 0.40 |
PCR reverse primer (10 µM) | 0.40 |
Marathon DNA polymerase (1 U/µL) | 1.00 |
ddH2O | 11.20 |
Total volume | 50 |
