Fig. 4 | Scientific Reports

Fig. 4

From: Tumor treating fields suppress tumor cell growth and induce immunogenic cell death biomarkers in biliary tract cancer cell lines

Fig. 4

TTFields application promotes DAMPs expression on BTC cells. Conditioned media from cells treated with control or 2.1 V/cm TTFields for 96 h were collected and assayed for biochemical markers of ICD. (A) Determination of LDH release in BTC cells after TTFields treatment (n = 5, ***p < 0.001). (B) Quantitative analysis of CRT surface exposure in live cells (PI-) was performed using a flow cytometer (n = 3, *p < 0.05,***p < 0.001). (C) Extracellular ATP in the culture medium of BTC cells was detected by fluorometric assay (n = 6, ***p < 0.001). (D) Soluble HMGB1 in the culture medium of BTC cells was detected by ELISA kit (n = 6, ***p < 0.001).

Back to article page