Fig. 2
Or56-Gal4(II) labels a group of poorly characterized LSO neurons. (a–a’’) Gr64f-LexA(KI) and Or56a-Gal4(II) label distinct populations of LSO neurons. Gr64f-LexA(KI) drove myr-GFP expression (a and a’’ green), while Or56a-Gal4(II) drove CD8-tdTomato expression (a’ and a’’ magenta). Scale bar = 20 µm. (b–b’’) ppk28-LexA(II) and Or56a-Gal4(II) label distinct populations of LSO neurons. ppk28-LexA(II) drove myr-GFP expression (b and b’’ green), while Or56a-Gal4(II) drove CD8-tdTomato expression (b’ and b’’ magenta). Scale bar = 20 µm. (c) mCD8-GFP expression driven by Ir60b-Gal4 labels one pair of LSO neurons. Scale bar = 20 µm. (d–d’) mCD8-GFP expression driven by both Ir60b-Gal4 and Or56a-Gal4(II) labels four pairs of LSO neurons. (d) shows the full confocal projection (64 µm), while (d’) shows a 26 µm stack to better visualize the cell bodies (labeled 1–4 on each side). (e) Measuring sucrose consumption time for individual flies using the temporal consumption assay. (f) Consumption time of control flies and flies with Or56a(II) LSO neurons silenced using tetanus toxin (TNT). All flies were food-deprived for approximately 24 h and tested with 300 mM sucrose. For box plots: whiskers = 10th–90th percentile, box = 25th–75th percentile, and line within box = median. Dots represent individual data points. N = 38–45 flies/genotype; one-way ANOVA followed by Tukey’s multiple comparison tests, ns = not significant. (g) Summary of the Or56a-Gal4(II) expression pattern in the LSO sensillum 7.
