Fig. 4
A knock-in Or56a-T2A-Gal4 driver does not drive expression in LSO neurons. (a) Schematic illustrating the generation of Or56a[MI07071]-T2A-Gal4 using the Trojan Exon approach (see text for details). P: attP site; B: attB site; SA: splice acceptor site; SD: splice donor site; pA, polyadenylation signal. (b, c) Immunostaining of mCD8-GFP expression driven by Or56a[MI07071]-T2A-Gal4 in two representative brains. Arrows indicate expression in the antennal lobes. (d, e) Immunostaining of mCD8-GFP expression driven by Or56a-Gal4(II) in two representative brains. Arrows indicate expression in the antennal lobes; arrowheads indicate expression in the dorsal subesophageal zone (SEZ), where LSO neurons project. In (b–e), immunostaining of mCD8-GFP expression is shown in black and white in the left panels and in green in the right panels. Immunostaining for Bruchpilot (Brp), which labels brain neuropils, is shown in magenta. Scale bars = 50 µm.
