Table 1 Surface antibody panel used for cytof analysis. IH: in-house conjugation, P: purchased pre-conjugated.
Marker | Clone | Isotope | Titration | Source (Conjugation method) |
|---|---|---|---|---|
CD19 | HIB19 | 142Nd | 1:50 | Standard Biotools, UK (P) |
CD14 | RMO52 | 148Nd | 1:200 | Standard Biotools, UK (P) |
CD3 | UCHT1 | 170Er | 1:200 | Standard Biotools, UK (P) |
CD16 | 3G8 | 209Bi | 1:200 | Standard Biotools, UK (P) |
HLADR | L243 | 174Yb | 1:100 | Standard Biotools, UK (P) |
CD5 | UCHT2 | 143Nd | 1:50 | Standard Biotools, UK (P) |
CD81 | 5A6 | 145Nd | 1:100 | Standard Biotools, UK (P) |
CD20 | 2H7 | 147Sm | 1:100 | Standard Biotools, UK (P) |
CD79b | 3A2-2E7 | 150Dy | 1:100 | ThermoFisher, UK (IH) |
ROR1 | 2A2 | 155Gd | 1:50 | Biolegend, UK (IH) |
CD43 | 1G10 | 168Er | 1:400 | ThermoFisher, UK (IH) |
CD38 | HIT2 | 144Nd | 1:100 | Standard Biotools, UK (P) |
IgD | IA6-2 | 146Nd | 1:100 | Standard Biotools, UK (P) |
CD24 | ML5 | 152Sm | 1:100 | Biolegend, UK (IH) |
CD27 | L128 | 158Gd | 1:100 | Standard Biotools, UK (P) |
sIgM | MHM-88 | 176Yb | 1:50 | Biolegend, UK (IH) |
CD138 | MI15 | 166Er | 1:100 | Biolegend, UK (IH) |
CD49d | 9F10 | 141Pr | 1:100 | Standard Biotools, UK (P) |
CD84 | CD84.1.21 | 154Sm | 1:100 | Standard Biotools, UK (P) |
CD180 | RP105 | 169Tm | 1:100 | Biolegend, UK (IH) |
CCR7 | G043H7 | 167Er | 1:100 | Standard Biotools, UK (P) |
CXCR4 | 12G5 | 175Lu | 1:100 | Standard Biotools, UK (P) |
CXCR5 | RF8B2 | 164Dy | 1:100 | Standard Biotools, UK (P) |
CD62L | DREG-56 | 153Eu | 1:100 | Standard Biotools, UK (P) |
