Fig. 1
From: RAD18 methylation by the methyltransferase SETD6 attenuates DNA breaks
SETD6 binds RAD18 in-vitro and in cells. (A) Schematic representation of the M-NAPPA process. DNA sequences encoding specific proteins were spotted onto a glass slide. The DNA is then expressed in-situ using a cell-free expression system, creating a protein microarray. The interaction was detected using fluorescence following data analysis. (B) Enzyme-linked immunosorbent assay (ELISA) was performed with the indicated recombinant proteins. Self-interaction between His-SETD6 and GST-SETD6 served as a positive control. The graph represents absorbance at 450 nm for each condition. ****p-value < 0.0001. (C) HeLa whole cell lysates were immunoprecipitated with agarose beads conjugated to the indicated antibody, followed by Western blot analysis with indicated antibodies.
