Table 1 Primers for cloning and mutagenesis.
From: RAD18 methylation by the methyltransferase SETD6 attenuates DNA breaks
Sequence 5’ to 3’ | |
|---|---|
RAD18 WT | |
RAD18 FW | TAAGGCGCGCCGACTCCCTGGCCGAGTCTC |
RAD18 REV | GGCTTAATTAATTAATTCCTATTACGCTTGTTTCTTGGTTC |
RAD18 mutants | |
RAD18 K73R FW | CAGAGCCGGATCTGcgcAATAACCGCATATTAGATGAACTGGTAAAAAGC |
RAD18 K73R REV | TAATATGCGGTTATTgcgCAGATCCGGCTCTGTGACAGTC |
RAD18 K406R FW | ACTTTTCTCAATCAcgcCTGGACTCCCCAGAGGAATTGG |
RAD18 K406R REV | TCTGGGGAGTCCAGgcgTGATTGAGAAAAGTGGTTTGTTACTGAGGTC |
