Fig. 2

The expression landscape of m6A RNA methylation regulators in IS and the construction of a random forest model to identify key m6A regulators. The box plot (A) and heatmap plot (B) reveal significant differences in the expression of eight m6A regulators between healthy and IS samples. (C), The influence of the number of decision trees on the error rate is demonstrated, with the x-axis representing the number of decision trees and the y-axis indicating the error rate. The error rate remains relatively stable at approximately 450 decision trees. (D), Results of the Gini coefficient method in the random forest classifier. The x-axis indicates the genetic variable, and the y-axis represents the importance index. *p < 0.05; **p < 0.01; ***p < 0.001.