Fig. 4

Unsupervised clustering of the eight key m6A regulators revealed the existence of two distinct m6A modification pattern subtypes in IS samples. (A) Consensus clustering cumulative distribution function (CDF) for k = 2–9. (B) Relative change in the area under the CDF curve for k = 2–9. (C) Heatmap of the matrix of cooccurrence proportions for IS samples. (D) Principal component analysis (PCA) for the transcriptome profiles of two m6A subtypes, showing a remarkable difference in the transcriptome between different modification patterns.