Fig. 1
Visual Workflow of the Immunogenicity Mitigation Strategy for Streptokinase. This diagram illustrates the in-silico methodology employed in this study, beginning with the retrieval of the Streptokinase sequence from UniProtKB, and progressing through the different steps involving computational tools and analyses, including: antigenicity prediction; 3D structure prediction with refinement; docking of streptokinase to plasminogen; linear and conformational B-cell epitope prediction; hot-spot residue determination; candidate hotspot alterations and evaluation; selection of effective point mutations; mutein 3D structure modeling, and validation. The diagram was generated with the assistance of Napkin website (at https://napkin.ai), an AI-powered (Artifficial Intelligence-Powered) note-taking tool, and shows the interconnectedness of the different analyses and the flow of our methodology, highlighting key relationships between different parts of the study.
