Fig. 5
Hotspot Residues for Immunogenicity Reduction on the 3D Structure of Streptokinase. This figure displays the Streptokinase (SK) protein structure (gray) with the conformational B-cell epitopes highlighted in hot pink. The key residues that have been identified as “hot spots” for mutagenesis based on their structural characteristics, low conservation, and high antigenicity scores are highlighted in cyan. These residues represent potential targets for reducing the protein’s immunogenic potential through targeted point mutations, without compromising the structural stability of the protein, nor its binding to its natural substrate (plasminogen). The selection of these specific residues was a direct consequence of the application of multiple computational tools and analyses described in our methodology. This image was created using PyMOL version 3.0.0.
