Table 2 Physiological and phenotypic features of EP7T in comparison to its closest relatives Tundrisphaera lichenicola PT12T and Singulisphaera acidiphila MOB10T, n.o.: not observed.

From: Kueselia aquadivae gen. nov., sp. nov., the first member of the family Isosphaeraceae isolated from subsurface percolates

Characteristics

EP7T

Tundrisphaera lichenicola

P12T53

Singulisphaera acidiphila

MOB10T51

Sampling location

Hainich CZE, Thuringia

(Germany)

Nadym Region, Yamalo-Nenets

Autonomous Okrug (Russia)

Obukhovskoe, Yaroslavl

Region (Russia)

Sampled material

Percolate of drainage

collector

Oxic layer of peat

Acidic Sphagnum Peat

(pH 4.2)

Temperature range

(optimum) (°C)

10–25 (18–21)

4–28 (15–22)

4–33 (20–26)

pH Range (optimum)

6.0–9.0 (7.5)

4.5–6.8 (5.5–6.0)

4.2–7.5 (5.0–6.2)

Relation to oxygen

Facultatively anaerobic

Aerobic

Aerobic

Cell shape

Spherical

Spherical

Spherical

Pigmentation

Light pink

Pink

Colorless (opaque)

Motility

Non-motile

Non-motile

Non-motile

Stalks

n.o.

Holdfast-like appendages

Absent

Aggregates

Forms small, microscopic

aggregates

Forms small chains

Forms shapeless aggregates

Cell division mode

Asymmetric cell division

(“polar” budding)

Asymmetric cell division

(“polar” budding)

Asymmetric cell division

(“polar” budding)