Fig. 3
From: Histamine as a mediator of cross-talk between human lung mast cells and macrophages
Effect of varying concentrations of histamine, alone and in combination with cetirizine, on cytokine release from human lung macrophages (HLMs) (A, C). HLMs were preincubated (30 min at 37 °C) with increasing concentrations of cetirizine (10−7- 10−5 M) and then incubated (18 h at 37 °C) with varying concentrations of histamine (3 × 10−6- 3 × 10−3 M). TNF-α and IL-1β concentrations in supernatants were evaluated by ELISA and expressed as ng of mediators for mg of total proteins. (B-D) In three independent experiments, the concentration of histamine, alone and in combination with cetirizine, needed to produce 50% of maximal effect was calculated. For each concentration of cetirizine, the dose ratio was calculated63. The KD was obtained from the value of log (cetirizine) where log (dose-ratio-1) = 063. The different symbols (●, ○, ◻) refer to the results from three experiments obtained from different donors.
