Fig. 4
From: Histamine as a mediator of cross-talk between human lung mast cells and macrophages

Effect of histamine on reactive oxygen species (ROS) production from human lung macrophages (HLMs). (A, B) 2′,7′-dichlorodihydrofluorescein diacetate-labeled HLMs were incubated (30 min at 37 °C) with complete medium (CTR) or increasing concentrations of histamine (10−7−10−5 M). Fluorescence was measured for 30 min at 2-min intervals. Data are normalized on T0 and are mean ± SD of three independent experiments with cells from different donors. ** p < 0.01 vs. CTR.