Fig. 6
From: Histamine as a mediator of cross-talk between human lung mast cells and macrophages
Effect of histamine on chemotaxis of human lung macrophages (HLMs). HLMs (1.8 × 105 cells/mL in 75 µL) were allowed to migrate (60 min at 37 °C) toward complete medium (CTR), increasing concentrations of histamine (10−6 −10−4 M) or FBS (235 µL per well). At the end of incubation, the cells were centrifuged and resuspended in phosphate-buffered saline (PBS) (100 µL) and counted by flow cytometry. Graphs show raw data of HLMs (counts/mL) (A) and migratory cells normalized for control medium (B). Data are the mean ± SD of three independent experiments obtained from different donors. * p < 0.05 vs. CTR.
