Fig. 4 | Scientific Reports

Fig. 4

From: Peroxiredoxins as novel indicators in multiple myeloma associated with ferroptosis and immune infiltration

Fig. 4

The expression and function of PRDX2 in MM. (A) Relative mRNA expression of PRDX2 in MM and HD. (B) PRDX2 in MM (n = 5) and HD (n = 2) were detected. T: MM, N: HD. (C, D) The expression and quantitative analysis of PRDX2 in myeloma cell lines (RPMI-8226, AMO1, MM1.S, and U-266). Relative quantification was normalized to U-266 as the reference. Data shown represent mean ± SD (N = 3). (E, F) Transfection effects of two different sequences were detected by qRT-PCR and WB in RPMI-8226 and AMO1 cells. Data shown represent mean ± SD (N = 3). (G, H) Results of CCK8 after targeting PRDX2 in RPMI-8226 and AMO1 cells (N = 3). (I, J) Invasion and migration of MM cells measured by Transwell assay. Scale bar: 100 μm. Data shown represent mean ± SD (N = 3). Full-length blots are presented in Supplementary Fig. 4.

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