Fig. 2
Analysis of Capn3-mRNA expression and CAPN3 western blot in WT and Capn3-deficient mice. (a) No significant differences in expression of Capn3-mRNA between female WT and Capn3-deficient mice were detected at any timepoint, but Capn3-deficient mice showed a high variance in mRNA expression. (b) Male Capn3-deficient mice showed significantly decreased expression of Capn3-mRNA at an age of 1.5 months (p = 0.028) and 15 months (p = 0.006). (c,d) The expression of whole-length 94 kDa CAPN3 protein was significantly reduced in both, female and male Capn3-deficient mice during the disease. (e,f) Examples of CAPN3 western blot of gastrocnemius and soleus muscles of female (e) and male (f) WT (sample 1–4) and Capn3-deficient (sample 5–8) mice at the age of 3 months. Asterisks indicate significance comparing genotypes within timepoints. See Supplementary Figure S3 for full-length western blots.
